CYP2C9 polymorphisms and CYP2C9*2 genotyping primers

Pyrosequencing method for genotyping cytochrome P450 CYP2C8 and CYP2C9 enzymes.

data show that Greiner lithium-heparin tubes produce a frequency of duplicate errors similar to the frequency of errors obtained with the BD tubes. From the data obtained, use of the modified Modular P IFCC method substantially reduced but did not totally eliminate duplicate errors. For many years, we have observed on an intermittent basis duplicate errors when using lithium-heparin gel tubes (...

frequency of single nucleotide polymorphisms of cytochrome p450 cyp2c9 in an iranian population using taqman genotyping assay

Multiplex genotyping of PCR products with MassTag-labeled primers.

A simple mass spectrometric based assay, the PinPoint assay, has previously been described for typing single nucleotide polymorphisms. The identity of a polymorphism is determined by mass differences of single base extended genotyping primers as determined by MALDI-TOF mass spectrometry. A simple method for multiplexing the assay is described, employing multiple primers with 5'oligo(dT) sequenc...

Genotyping of essential hypertension single-nucleotide polymorphisms by a homogeneous PCR method with universal energy transfer primers.

BACKGROUND Human hypertension is a complex, multifactorial disease with a heritability of more than 30-50%. A genetic screening test based on analysis of multiple single-nucleotide polymorphisms (SNPs) to assess the likelihood of developing hypertension would be helpful for disease management. METHODS Tailed allele-specific primers were designed to amplify by PCR six biallelic SNP loci [three...

Clinical relevance of genetic polymorphisms in the human CYP2C9 gene.

Cytochrome p450 (CYP) 2C9 hydroxylates about 16% of drugs in current clinical use. Of special interest are those with a narrow therapeutic index, such as S-warfarin, tolbutamide and phenytoin, where impairment in CYP2C9 metabolic activity might cause difficulties in dose adjustment as well as toxicity. Single-nucleotide polymorphisms (SNP) in the CYP2C9 gene have increasingly been recognized as...